- Agarose LM, Low melting point (< 65°C)
- Agarose HR, PCR grade for DNA fragments between 20-800 bp
- Agarose 3:1, Blended agarose for small fragments
Specifications
| Agarose LM | Agarose HR | Agarose 3:1 | |
|---|---|---|---|
| Gel Strength: | > 200g/cm² (1%) | > 750g/cm² (1.5%) | > 650g/cm² (1.5%) |
| Gel Temperature: | 26°C to 30°C | ≤ 33°C | ≤ 36°C |
| Melt temperature: | ≤ 65°C | ≤ 70°C | ≤ 80°C |
| EEO (-mr): | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% |
| Moisture Content: | ≤ 10% | ≤ 10% | ≤ 10% |
| Sulfate: | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% |
| RNase / DNase: | None Detected | None Detected | None Detected |
| Protease / Endonuclease: | None Detected | None Detected | None Detected |
| Storage Conditions: | Room Temperature | Room Temperature | Room Temperature |
| Resolution: | 200bp to 25kbp | 20bp to 800bp | 50bp to 1kb |
| Applications: | Prep gels In Gel Processing | AFLP’s STR’s PCR Small Fragments | PCR Blotting |
Benchmark’s specialty agarose is the perfect choice when resolving small DNA/RNA fragments, isolating nucleic acids from gels or performing in-gel manipulations. Available in three grades, LM, HR and 3:1, it will meet all your electrophoretic needs.
Agarose LM – this low melt agarose can be used to resolve samples from 200bp to 25kb. It is perfect for prep gels as it has a melting point well below denaturation temperatures (65°C). The agarose remains liquid at 37°C, allowing for procedures such as restriction digests and labeling to be carried out in the gel.
Agarose HR – for applications such as analysis of STR’s, AFLP’s, and PCR products where separation of small fragments is required, Benchmark’s Agarose HR is ideal. Clear resolution of 20bp to 800bp fragments can be carried out in this intermediate melting agarose.
Agarose 3:1 – this blended high resolution agarose is designed for separation of fragments less than 1,000bp. Gels made with Agarose 3:1 are suitable for analytical and blotting applications.
All Benchmark agarose is manufactured under a “green” process that eliminates the use of organic solvents. They provide gels with enhanced resolution and clarity, low EEO and low backgrounds. All the agarose is tested to be RNase, DNase, Protease and Endonuclease free.
